Both parents were from the Chickacoan (sometimes spelled Chickahominy/Chickacoen), a subtribe of the Powhatan Confederacy that received a 4,400-acre reservation in 1648 near the Rappahannock River. The tribe largely disappeared from records after smallpox epidemics and colonial land encroachment by the early 1700s.
This places Mary Ann as 100% Powhatan through both lines, explaining her “Little Flower” nickname tied to Native heritage. Records become speculative beyond her parents due to limited 17th-century documentation.